FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389168
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC9380.24102701147062452Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC6750.17344694322246434Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC6600.16959256670640957No Hit
AGTGAGTCGTATTACCGGCC5790.14877893351971386No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5050.1297640093738437Clontech Universal Primer Mix Short (95% over 21bp)
CGTGCCCCTATAGTGAGTCGTATTACCGGCC4290.11023516835916622Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4230.10869341775274431Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC3950.1014985815894421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)