FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403829
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC9390.23252416245490046Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC7190.17804565793937535No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC6800.1683881048661686Clontech Universal Primer Mix Short (95% over 21bp)
AGTGAGTCGTATTACCGGCC6150.1522921830774907No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5500.13619626128881285Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC4410.10920463859702993No Hit
CGTGCCCCTATAGTGAGTCGTATTACCGGCC4370.1082141203331113Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4040.10004234465578253Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)