FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395905
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC11700.295525441709501Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA10240.25864790795771714No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC8230.20787815258711054Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC5720.14447910483575604Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC4780.12073603515994999Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA4780.12073603515994999No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT4510.11391621727434613Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATA4360.11012742956012175No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC4200.1060860559982824Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACT4140.10457054091259266No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT4120.10406536921736276Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)