FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395905
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC9360.23642035336760078Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC6720.16973768959725186Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC6650.16796958866394715No Hit
AGTGAGTCGTATTACCGGCC5730.144731690683371No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5200.13134464075977823Clontech Universal Primer Mix Short (95% over 21bp)
CGTGCCCCTATAGTGAGTCGTATTACCGGCC4350.10987484371250678Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC4190.10583347015066745No Hit
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4090.10330761167451788Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)