FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205126
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5850.2851905657985823Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC3740.18232696001482016No Hit
GGCCGGTAATACGACTCACTATA3600.17550188664528144No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3300.16087672942484132Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA2800.13650146739077446No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC2380.1160262472821583Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC2200.10725115294989421Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2190.10676364770921287Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2100.10237610054308083Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)