FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205126
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4750.23156498932363523Clontech Universal Primer Mix Short (95% over 21bp)
GTATTACCGGCC3190.1555141717773466No Hit
TATTACCGGCC3130.15258914033325857No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2520.122851320651697Clontech Universal Primer Mix Short (95% over 21bp)
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2480.12090129968897166Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2320.11310121583807027Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2160.10530113198716885Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)