FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005711452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202215
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4790.23687659174640852Clontech Universal Primer Mix Short (95% over 21bp)
GTATTACCGGCC3120.15429122468659595No Hit
TATTACCGGCC2810.13896100684914572No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2330.1152238953589002Clontech Universal Primer Mix Short (95% over 21bp)
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2280.11275127957866626Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2110.10434438592587097Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC2080.10286081645773062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)