FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205678
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4690.22802633242252454Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC3330.16190355798870076No Hit
GTATTACCGGCC3060.1487762424761034No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2730.13273174573848442Clontech Universal Primer Mix Short (95% over 21bp)
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2300.11182528029249604Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2190.10647711471328969Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)