FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204235
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6070.29720664920312384Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC3590.17577790290596618No Hit
GGCCGGTAATACGACTCACTATA3510.17186084657379977No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3080.1508066687884055Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA2530.12387690650476166No Hit
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2450.11995985017259529Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC2280.11163610546674174Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)