FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005711456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204235
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4920.24089896442823217Clontech Universal Primer Mix Short (95% over 21bp)
GTATTACCGGCC3160.1547237251205719No Hit
TATTACCGGCC2900.14199329204103117No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2270.11114647342522095Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2250.11016720934217934Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)