FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428098
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC12570.2936243570397432Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC6950.16234600488673154No Hit
GGCCGGTAATACGACTCACTATA6920.1616452307649183No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC5890.13758531924933076Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA5350.12497138505669263No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC4640.10838639750711286Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC4330.10114506491504281Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)