FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428098
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC9320.21770716050997668Clontech Universal Primer Mix Short (95% over 21bp)
GTATTACCGGCC6410.14973207069409342No Hit
TATTACCGGCC5720.13361426589238912No Hit
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4430.10348097865442024Clontech Universal Primer Mix Short (95% over 21bp)
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4360.10184583903685605Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4330.10114506491504281Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)