FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444815
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC12510.28124051572001846Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA7070.15894248170587771No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC6940.1560199183930398Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC6940.1560199183930398No Hit
GGCCGGTAATA5280.11870103301372481No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC4660.10476265413711318Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC4660.10476265413711318Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)