FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437148
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC13180.301499720918316Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA7360.16836403231857403No Hit
GGCCGGTAATAC6600.1509786159378517No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC6190.1415996413114094Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA5350.12238418110113737No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC4480.10248245445478418Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC4420.1011099215826219Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)