FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437148
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC9940.22738294582155244Clontech Universal Primer Mix Short (95% over 21bp)
GTATTACCGGCC6440.14731852827875228No Hit
TATTACCGGCC5560.12718804615370538No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4770.10911636333690192Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4610.10545627567780248Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4520.10339747636955905Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)