FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902922
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC83670.9266581166479496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC56840.6295117407705206TruSeq Adapter, Index 11 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA53150.5886444233278179TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG23240.2573865738125774TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19600.21707301405880022Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG19360.21441497715195776TruSeq Adapter, Index 2 (100% over 35bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT18160.2011247926177455TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA13090.14497376296069872TruSeq Adapter, Index 2 (97% over 35bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT11270.12481698308381012TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA10070.11152679854959785TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9360.10366343936685561Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)