FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914718
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC88340.9657621255949921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC58990.6448982090655262TruSeq Adapter, Index 11 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA54340.5940628696494439TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG23190.25352075721697837TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG20880.2282670724747955TruSeq Adapter, Index 2 (100% over 35bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20560.22476872653648447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT17890.19557940261370169TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA14560.15917474019315242TruSeq Adapter, Index 2 (97% over 35bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT11800.12900150647521968TruSeq Adapter, Index 22 (97% over 37bp)
GGATGGCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9560.10451308490704238Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)