FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977203
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG511322.586077403281302TruSeq Adapter, Index 5 (97% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG332891.6836409817302525TruSeq Adapter, Index 15 (97% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA137340.6946175986987679TruSeq Adapter, Index 25 (97% over 38bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC126520.6398938298191941Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA100690.5092547401556643Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA88710.4486640977178368TruSeq Adapter, Index 25 (97% over 38bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG75710.3829146526684412TruSeq Adapter, Index 15 (97% over 36bp)
CTGGTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC37330.18880206028414886TruSeq Adapter, Index 5 (100% over 35bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC37220.1882457188260386TruSeq Adapter, Index 5 (100% over 35bp)
CTGGTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC27240.13777037562657957Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23850.12062494341754489Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA19860.10044492143699965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)