FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007028
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG528402.6327485216947646TruSeq Adapter, Index 5 (97% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG344821.7180627275753004TruSeq Adapter, Index 15 (97% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA143360.7142899849927355TruSeq Adapter, Index 25 (97% over 38bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC130870.6520586658482094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA105750.5268984787456876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA85150.42425915333518016TruSeq Adapter, Index 25 (97% over 38bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG80610.4016386418126703TruSeq Adapter, Index 15 (97% over 36bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC38810.1933704960767862TruSeq Adapter, Index 5 (100% over 35bp)
CTGGTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC38490.19177609878885596TruSeq Adapter, Index 5 (100% over 35bp)
CTGGTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC29540.14718279964205783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24530.1222205171029004Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA21660.10792076642677632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)