FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510846
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG311016.088136150620735TruSeq Adapter, Index 5 (97% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG192323.7647353605587597TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA119522.339648348034437TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA118802.325554080877603TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC102061.9978623694812137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG47020.9204339468254621TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC40050.7839936105988888TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC33990.6653668620288697TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA31400.6146666510063699Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC21330.41754266452120603TruSeq Adapter, Index 5 (97% over 35bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19180.37545561676121575Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG17240.33747939692196866TruSeq Adapter, Index 5 (97% over 37bp)
GGTCTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA15720.3077248329242081TruSeq Adapter, Index 5 (97% over 35bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9550.18694479353856153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC9470.18537876385446886TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG9310.18224670448628355TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC8830.17285052638172757TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC6850.13409129170043418TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6470.12665265070099405Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTCTTGGTTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6180.12097579309615814Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG5660.11079660014955584TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTACCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5320.10414097399216202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5170.10120466833448828Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)