FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520317
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG318556.122229333271832TruSeq Adapter, Index 5 (97% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG199723.838429265236385TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA119192.2907189271155852TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA116162.2324851965244266TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC106212.041255619170621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG48260.9275114977984574TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC40720.7825998381755737TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA32020.6153940770722464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC31600.6073220748120857TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC24290.46683079737928995TruSeq Adapter, Index 5 (97% over 35bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20160.3874561084877104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA18450.3545915278570564TruSeq Adapter, Index 5 (97% over 35bp)
GGTCTTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG16980.3263395199464942TruSeq Adapter, Index 5 (97% over 37bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10390.19968596067397376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC9240.17758404972353392TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG9210.17700747813352247TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC8940.17181833382341918TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC6790.1304973698725969TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGGTTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6530.12550041609249746Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6510.1251160350324898Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTCTTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG5820.11185488846222591TruSeq Adapter, Index 15 (97% over 36bp)
GGTCTTGGTTTTACCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5210.10013126613199262Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)