FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959067
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG433404.518975212367853TruSeq Adapter, Index 12 (100% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT220602.3001521270151097TruSeq Adapter, Index 12 (100% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC138941.4486996216114203Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT114891.197935076485793TruSeq Adapter, Index 12 (100% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC105361.0985676704547231Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTC81870.8536421334484453TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT52050.5427149510930935TruSeq Adapter, Index 12 (100% over 36bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT37080.3866257519026304TruSeq Adapter, Index 1 (97% over 35bp)
CTGGTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26750.27891690570106153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC24850.25910598529612633TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC23760.24774077306382142TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT22250.2319963047420045TruSeq Adapter, Index 1 (97% over 35bp)
CTGGTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21150.2205268245075683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC18050.1882037438468845TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC16970.17694279961671083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC14850.15483798316488837TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC13420.13992765886012135TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCG12440.12970939465126002TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC12310.12835391062355392TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT12160.12678989059158535TruSeq Adapter, Index 12 (100% over 36bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC11780.12282770651059832TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC11370.11855271842321756TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC11250.11730150239764271TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT10860.11323505031452442TruSeq Adapter, Index 12 (97% over 38bp)
CTGGTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTC10280.10718750619091262TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)