FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900538
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG500172.6317284895119175TruSeq Adapter, Index 12 (100% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT327051.720828523291826TruSeq Adapter, Index 12 (100% over 36bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC243711.2823211111800974Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT191761.0089774579619035TruSeq Adapter, Index 12 (100% over 38bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTC186200.9797225838157406TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC88260.4643948187302753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT79550.4185656903466281TruSeq Adapter, Index 12 (100% over 36bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT50540.26592470132141527TruSeq Adapter, Index 1 (97% over 35bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC44900.23624889373430052Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCG41630.2190432393353882TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCG40360.21236092096027548TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC35910.1889464983073214TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTC33780.17773914544197486TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC31400.16521637557365335TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC30920.16269077492794146TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC29300.15416687274866378TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC28220.14848427129581204TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT25090.1320152504185657TruSeq Adapter, Index 1 (97% over 35bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23990.12622741560547593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC23410.12317564815857406TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG22270.11717734662500828TruSeq Adapter, Index 12 (100% over 37bp)
GGTCTTGGTTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT21640.11386249577751142TruSeq Adapter, Index 1 (97% over 35bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT21230.11170521189263251TruSeq Adapter, Index 12 (97% over 38bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC20030.10539121027835276TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)