FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406317
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACCTCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA238271.6942837212378148Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC76170.5416275277906759TruSeq Adapter, Index 5 (100% over 35bp)
TAACCTCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG31410.22334935864389038TruSeq Adapter, Index 5 (97% over 37bp)
TAACCTCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG24480.17407170645025266TruSeq Adapter, Index 15 (97% over 36bp)
TAACCTCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23710.1685964117620707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCTCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA21450.15252606631364052TruSeq Adapter, Index 25 (97% over 38bp)
TAACCTCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA16830.11967429818454872TruSeq Adapter, Index 25 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)