FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155815
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT112820.9761077681116788TruSeq Adapter, Index 12 (100% over 36bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG89180.7715767661779783TruSeq Adapter, Index 12 (100% over 37bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC72930.630983332107647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC40070.34668177865834926Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT34790.3009997274650355TruSeq Adapter, Index 1 (97% over 35bp)
TCCATTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT34740.30056713228328064TruSeq Adapter, Index 12 (100% over 36bp)
TCCATTGGTTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC19150.16568395461211352Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCAATGGAGATCGGAAGAGCACACGTCTGAACTCCAG16960.1467362856512504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCG15910.13765178683439822TruSeq Adapter, Index 12 (97% over 38bp)
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC15850.13713267261629242TruSeq Adapter, Index 12 (97% over 38bp)
TCCATTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT15830.13695963454359045TruSeq Adapter, Index 12 (100% over 36bp)
TCCATTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT15780.13652703936183558TruSeq Adapter, Index 12 (100% over 38bp)
TCCATTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14170.12259747450932891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13630.11792544654637636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT13530.11706025618286664TruSeq Adapter, Index 1 (97% over 35bp)
TCCATTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCG13150.11377253280152966TruSeq Adapter, Index 12 (97% over 38bp)
TCCATTGGTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG12840.11109044267464949TruSeq Adapter, Index 12 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)