FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501127
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA60051.1982990339774149Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG47450.9468657645666668TruSeq Adapter, Index 15 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG38550.7692660742685986TruSeq Adapter, Index 5 (97% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC13050.2604130290325606TruSeq Adapter, Index 5 (100% over 35bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12160.24265306000275377Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA11230.22409489011767478TruSeq Adapter, Index 25 (97% over 38bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA9340.18637989970606253TruSeq Adapter, Index 25 (97% over 38bp)
GGATGGCCTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC7330.14627030672863364TruSeq Adapter, Index 5 (97% over 35bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC5440.10855531631702142TruSeq Adapter, Index 25 (97% over 38bp)
GGATGGCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5090.10157105883338954Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)