FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506921
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA63531.2532524791831468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG47050.9281525129162138TruSeq Adapter, Index 15 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG39380.7768468854121253TruSeq Adapter, Index 5 (97% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC13260.2615792204307969TruSeq Adapter, Index 5 (100% over 35bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12240.2414577419361202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA10640.20989463841505876TruSeq Adapter, Index 25 (97% over 38bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA9990.19707212760962756TruSeq Adapter, Index 25 (97% over 38bp)
GGATGGCCTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC7960.15702644001728078TruSeq Adapter, Index 5 (97% over 35bp)
GGATGGCCTGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5180.10218554764943649Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)