FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020412
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT91080.8925806438967789Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC87980.8622007581251494TruSeq Adapter, Index 18 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC87730.8597507673371148TruSeq Adapter, Index 1 (97% over 36bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT37580.36828261525736666TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCA26160.25636703605994443TruSeq Adapter, Index 18 (97% over 37bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAA23270.22804514255026404TruSeq Adapter, Index 18 (97% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAGGA11850.1161295633528418TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)