FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965388
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA71350.7390810741380668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG54060.5599821004611617TruSeq Adapter, Index 5 (97% over 37bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG51560.5340857769104236TruSeq Adapter, Index 15 (97% over 36bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA29990.3106522973146548TruSeq Adapter, Index 25 (97% over 38bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC24270.2514015090305659TruSeq Adapter, Index 5 (100% over 35bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23550.24394336784795337Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC16510.17101932072907472Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9680.10027056478845811Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)