FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976780
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA76310.7812404021376359Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG54100.5538606441573333TruSeq Adapter, Index 5 (97% over 37bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG52960.5421896435225946TruSeq Adapter, Index 15 (97% over 36bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA31150.3189049734843056TruSeq Adapter, Index 25 (97% over 38bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC26470.27099244456274696TruSeq Adapter, Index 5 (100% over 35bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24790.2537930752062901Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17610.18028624664714674Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)