FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968376
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG36710.3790882880203557TruSeq Adapter, Index 15 (97% over 36bp)
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA30380.3137211165910762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC25760.2660123753583319TruSeq Adapter, Index 5 (100% over 35bp)
TAGAACTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG17540.18112799160656604TruSeq Adapter, Index 5 (97% over 37bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13820.14271316100357712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA10950.11307591266202384Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)