FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044669
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC42120.4031899099140493Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC38390.3674848205508156TruSeq Adapter, Index 11 (97% over 36bp)
TAGAACTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG34920.3342685577919896TruSeq Adapter, Index 2 (100% over 35bp)
TAGAACTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA21600.20676405636617917TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC18150.1737392418076922Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12760.12214395181631695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA12520.11984657341224828TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11990.11477319610326334Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGAACTGGCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC11370.10883830189275262TruSeq Adapter, Index 11 (97% over 36bp)
TAGAACTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT11240.10759388859054876TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC11190.10711526808970115Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCCAGTTCTAGATCGGAAGAGCACACGTCTGAACTCC10750.10290340768224193Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)