FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056774
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC43550.41210325007996035Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC39690.37557699186391796TruSeq Adapter, Index 11 (97% over 36bp)
TAGAACTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG36160.3421734448425113TruSeq Adapter, Index 2 (100% over 35bp)
TAGAACTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA22600.21385840302656955TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC18670.17666975152681652Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13700.12963982838336296Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC12750.12065020524729034TruSeq Adapter, Index 11 (97% over 36bp)
TAGAACTGGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA12540.1186630253961585TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12190.11535105897760543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGAACTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC11570.10948414703616856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT11300.10692920151328478TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTGGCTTACCCAGTTCTAGATCGGAAGAGCACACGTCTGAACTCC10660.1008730343479306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)