FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152666
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA94280.8179299120473753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG56150.48713157150466824TruSeq Adapter, Index 5 (97% over 37bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA51260.44470818086071773TruSeq Adapter, Index 25 (97% over 38bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC29250.2537595452628949Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG28370.24612507005498557TruSeq Adapter, Index 15 (97% over 36bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA23690.20552354281292237TruSeq Adapter, Index 25 (97% over 38bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC16120.1398497049448843TruSeq Adapter, Index 5 (100% over 35bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15610.13542517954030048Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTACCTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA14130.12258538032699845TruSeq Adapter, Index 25 (97% over 38bp)
TTACCTTGACTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12970.11252175391657253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA12310.10679589751064054TruSeq Adapter, Index 25 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)