FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820825
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC49380.6015898638564858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG26460.3223586026254074TruSeq Adapter, Index 5 (97% over 37bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC16250.19797155301069044TruSeq Adapter, Index 5 (100% over 35bp)
CATTACTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG15470.18846891846617733TruSeq Adapter, Index 15 (97% over 36bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA15190.1850577163219931Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA9700.11817378856638137TruSeq Adapter, Index 25 (97% over 38bp)
CATTACTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA8490.10343252215758536Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)