FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271144
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC156281.2294437136941212TruSeq Adapter, Index 1 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC113270.8910870837607698TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC99930.7861422466691421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCA91220.7176212923162127TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT70480.5544611782772054Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT51110.4020787574027805TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAA50980.4010560565915427TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC47440.37320712680860707TruSeq Adapter, Index 1 (97% over 36bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC36750.289109652407595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT32790.2579566123114297TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT29360.23097304475338748TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT20010.15741725563744155Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT16450.12941098726816158TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14860.11690256965379217Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA14290.1124184199429805Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)