FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290570
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC162331.2578163137218439TruSeq Adapter, Index 1 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC118920.921453311327553TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC102050.7907358763957012Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCA93120.7215416443896883TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT73610.5703681319107061Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT54450.4219065994095632TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAA49520.3837064242931418TruSeq Adapter, Index 18 (97% over 37bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC49230.38145935516864643TruSeq Adapter, Index 1 (97% over 36bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38820.30079732211348476Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT35550.27545968060624376TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT29590.22927853584075253TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT21030.16295125409702688Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT16560.1283153955229085TruSeq Adapter, Index 1 (97% over 35bp)
GAGGCTTGGTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15670.1214192178649744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA14970.11599525790929588Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)