FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999450
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAGATAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC101060.505438995723824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATAAGATAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC56170.28092725499512367Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATAAGATAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG32680.16344494736052415TruSeq Adapter, Index 2 (100% over 35bp)
GATAAGATAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC30680.1534421966040661TruSeq Adapter, Index 11 (97% over 36bp)
TAGAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT28900.14453974843081846TruSeq Adapter, Index 22 (97% over 37bp)
TAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATA26840.1342369151516667TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)