FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185260
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC171711.448711675075511Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA163851.3823971111823568TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC158031.3332939608187233TruSeq Adapter, Index 11 (97% over 36bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG66300.5593709397094309TruSeq Adapter, Index 2 (100% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG56960.48056966403995743TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT53190.4487622968800094TruSeq Adapter, Index 22 (97% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATA22900.1932065538362891TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA18930.1597117931930547TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG14820.12503585711151982TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14510.12242039721242597Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13820.11659888969508801Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA12150.10250915410964682TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC12090.10200293606466092TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)