FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202156
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC176931.4717723822864919Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA168331.400234245805037TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC164771.3706207846568996TruSeq Adapter, Index 11 (97% over 36bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG69620.579126169981267TruSeq Adapter, Index 2 (100% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG56120.4668279324813086TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT53990.44910976612020403TruSeq Adapter, Index 22 (97% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATA21520.17901170896289667TruSeq Adapter, Index 22 (97% over 37bp)
GCTTGCCATATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA21040.17501888274067592TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG18040.15006371885179629TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15270.1270217841943974Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14660.12194756753699186Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC12660.10531079161107211TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)