FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614353
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC123760.47338672321603087TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT97460.37278821949446TruSeq Adapter, Index 12 (100% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT90640.3467014592138093TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAA71240.27249571882603457TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC58550.22395598452083554TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG57960.22169921200388779TruSeq Adapter, Index 12 (100% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC54870.20987984407614427Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCGC54650.20903833567999425TruSeq Adapter, Index 12 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC48910.18708261661680728TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT44770.17124695861652958TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC38460.14711096779968122TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC28370.10851633272170973TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)