FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645763
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC134530.5084733591028372TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT104490.39493333303096306TruSeq Adapter, Index 12 (100% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT93240.35241251767448556TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAA66430.2510806901449601TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC63720.2408378981790886TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG59700.2256437934917073TruSeq Adapter, Index 12 (100% over 37bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCGC57590.217668778344848TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC56170.2123017065398526Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC50840.19215628913096144TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT46890.17722675840579824TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC40860.1544356013747263TruSeq Adapter, Index 12 (97% over 38bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC28420.1074170286605414TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)