FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612055
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC82580.5122654003740567Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT63750.39545797134713145TruSeq Adapter, Index 12 (100% over 36bp)
TAGAACTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT59780.37083102003343554TruSeq Adapter, Index 1 (97% over 35bp)
TAGAACTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG45300.2810077819925499TruSeq Adapter, Index 12 (100% over 37bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC33310.20663066706781097Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC24450.15166976312842925Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19370.12015719066657155Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC17900.11103839509197888TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)