FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619560
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA275411.700523599002198TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC182051.1240707352614292TruSeq Adapter, Index 11 (97% over 36bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG148860.9191385314529873TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC148690.9180888636419767Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT127870.7895354293758798TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA54210.33472054138160984TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC45400.2803230507051298TruSeq Adapter, Index 11 (97% over 36bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC42970.26531897552421646Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG37010.22851885697349897TruSeq Adapter, Index 2 (100% over 35bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA32250.19912815826520783TruSeq Adapter, Index 2 (97% over 35bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25980.16041393958853023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA20060.12386080169922696TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG19950.12318160488033786TruSeq Adapter, Index 2 (97% over 35bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC16260.10039763886487688Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)