FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641478
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA288611.7582325197169866TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC192341.1717488750991485TruSeq Adapter, Index 11 (97% over 36bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC154600.9418341275362813Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG152390.928370651327645TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT129450.7886185498678631TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA52960.3226360633526615TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC47740.290835454389276TruSeq Adapter, Index 11 (97% over 36bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC43650.2659188852972748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA38710.23582405612502877TruSeq Adapter, Index 2 (97% over 35bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG35950.21900994104093993TruSeq Adapter, Index 2 (100% over 35bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26680.1625364458128589Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG23860.1453568064878116TruSeq Adapter, Index 2 (97% over 35bp)
GGTCTTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA19290.11751604346814273TruSeq Adapter, Index 22 (97% over 37bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17480.10648939553256273Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)