FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568493
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC79630.507684764930414TruSeq Adapter, Index 11 (97% over 36bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58090.3703554940952876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA57600.36723147632791475TruSeq Adapter, Index 22 (97% over 37bp)
TCCATTGGTTTTACCAATGGAGATCGGAAGAGCACACGTCTGAACTCCAG33200.21166814260567307Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC30540.19470918901136314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC25460.16232141297410954TruSeq Adapter, Index 11 (97% over 36bp)
TCCATTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG23400.14918778725821535TruSeq Adapter, Index 2 (100% over 35bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA20780.13248385552246647TruSeq Adapter, Index 22 (97% over 37bp)
TCCATTGGTTTTACCCAATGGAGATCGGAAGAGCACACGTCTGAACTCCA17070.10883057814092889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)