FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588464
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC82750.5209435026541362TruSeq Adapter, Index 11 (97% over 36bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA60940.38364105198481047TruSeq Adapter, Index 22 (97% over 37bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58140.3660139606563321Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCAATGGAGATCGGAAGAGCACACGTCTGAACTCCAG33600.21152509594173996Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC31880.20069702555424612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC27090.17054210860302782TruSeq Adapter, Index 11 (97% over 36bp)
TCCATTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG24190.15228547829853242TruSeq Adapter, Index 2 (100% over 35bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA19910.12534121012500127TruSeq Adapter, Index 22 (97% over 37bp)
TCCATTGGTTTTACCCAATGGAGATCGGAAGAGCACACGTCTGAACTCCA16860.10614027135648021Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)