FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379645
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG68481.8037903831210738TruSeq Adapter, Index 15 (97% over 36bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG53731.4152695281117886TruSeq Adapter, Index 5 (97% over 37bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC31020.8170791133822387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG24050.6334865466422579TruSeq Adapter, Index 15 (97% over 36bp)
TCCATTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC21190.5581530113658812TruSeq Adapter, Index 5 (100% over 35bp)
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA20150.5307589985381079Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC10230.2694622607962702TruSeq Adapter, Index 25 (97% over 38bp)
TCCATTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG9880.2602431218638465TruSeq Adapter, Index 15 (97% over 36bp)
TCCATTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA9730.2562920623213792TruSeq Adapter, Index 25 (97% over 38bp)
TCCATTGGTTTTACCAATGGAGATCGGAAGAGCACACGTCTGAACTCCAG9670.25471163850439227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATTGGTTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA9550.25155079087041843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8520.2244201820121429Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG8060.21230359941524318TruSeq Adapter, Index 5 (97% over 37bp)
TCCATTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7590.1999236128488456Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC7560.19913340094035217TruSeq Adapter, Index 5 (100% over 35bp)
TCCATTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC7340.19333851361140014TruSeq Adapter, Index 5 (97% over 35bp)
TCCATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA6630.17463683177705489TruSeq Adapter, Index 25 (97% over 38bp)
TCCATTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG6240.16436407696663988TruSeq Adapter, Index 5 (97% over 37bp)
TCCATTGGTTTTACCAATGAGATCGGAAGAGCACACGTCTGAACTCCAGT4840.12748752123694504Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCATTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC4760.1253802894809625TruSeq Adapter, Index 5 (100% over 35bp)
TCCATTGGTTTTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4620.12169263390799298Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA4490.11826838230452133TruSeq Adapter, Index 25 (97% over 38bp)
TCCATTGGTTTTAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4150.10931264734159545Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4060.10694201161611505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCCAATGGAGATCGGAAGAGCACACGTCTGAACTCCA3980.10483477986013248Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCATTGGTTTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC3830.10088372031766518TruSeq Adapter, Index 5 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)