FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162543
Sequences flagged as poor quality0
Sequence length10-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC113630.9774262113315378Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA87640.7538645882345857TruSeq Adapter, Index 22 (97% over 37bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT74930.6445352989093737TruSeq Adapter, Index 22 (97% over 37bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC72380.6226006263854327TruSeq Adapter, Index 11 (97% over 36bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG42640.3667821319297437TruSeq Adapter, Index 2 (100% over 35bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38400.33031036271346526Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC29740.25581849445568894Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG23740.20420750028171003TruSeq Adapter, Index 22 (97% over 37bp)
CATGGCTGGCTTACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16200.13934968426974315Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATGGCTGGCTTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC14210.12223203786870679Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12840.11044752753231495Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC12000.10322198834795789Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)