FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289626
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC95100.7374230978593793Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT61410.47618456823916394TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA53310.4133756608505101TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG44150.34234731619864983TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC36080.279771034392917Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT23260.18036236862470204TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC20050.15547143125216148TruSeq Adapter, Index 11 (97% over 36bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG17140.13290674970883032TruSeq Adapter, Index 2 (100% over 35bp)
TTACCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT16380.12701356827483318TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13270.10289804951202908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)